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  • KD/KO Validated

CD146/MCAM Polyklonaler Antikörper

CD146/MCAM Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(32)

Kat-Nr. : 17564-1-AP

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A375-Zellen, HepG2-Zellen, HUVEC-Zellen, Mauslungengewebe
Erfolgreiche IPHeLa-Zellen, HepG2-Zellen
Erfolgreiche Detektion in IHChuman ovary cancer tissue
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMausmilzgewebe
Erfolgreiche Detektion in IF/ICCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20000-1:80000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17564-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment CD146/MCAM und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Kaninchen, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD146/MCAM fusion protein Ag11762
Vollständiger Name melanoma cell adhesion molecule
Berechnetes Molekulargewicht 646 aa, 72 kDa
Beobachtetes Molekulargewicht113-120 kDa
GenBank-ZugangsnummerBC056418
Gene symbol CD146
Gene ID (NCBI) 4162
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CD146/MCAM antibody 17564-1-APProtokoll herunterladen
IHC protocol for CD146/MCAM antibody 17564-1-APProtokoll herunterladenl
IF protocol for CD146/MCAM antibody 17564-1-APProtokoll herunterladen
IP protocol for CD146/MCAM antibody 17564-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Biomaterials

Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Authors - W Benton Swanson
ratIF

Adv Healthc Mater

Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

Authors - Xiaoyi Wu
humanWB

Oncogene

The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.

Authors - M Sechler
  • KD Validated
humanIF

Cell Prolif

Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Authors - Lihua Luo
human

Stem Cell Res Ther

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Authors - Zuping Wu
ratWB

J Proteome Res

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.

Authors - Ji Yuhua Y
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognises a band around the expected molecular weight, however this band did not respond to deglycosylation treatment. This strongly suggests the recognised band is another protein other than Mcam, because Mcam is glycosylated, and looking at the other Mcam antibody from the same brand (66153-1-g), it is clear that the band is responsive to PNGaseF treatment.

  • Applications: Western Blot