MCCC2 Polyklonaler Antikörper

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• KD/KO Validated

MCCC2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12117-1-AP

Synonyme

MCCase subunit beta, MCCB, MCCC2

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• 

  WB analysis of human liver using 12117-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12117-1-AP (MCCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12117-1-AP

  IP result of anti-MCCC2 (IP:12117-1-AP, 4ug; Detection:12117-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 12117-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12117-1-AP (MCCC2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12117-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA MCCC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCCC2 fusion protein Ag2767
• Vollständiger Name: methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
• Berechnetes Molekulargewicht: 563 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC014897
• Gene symbol: MCCC2
• Gene ID (NCBI): 64087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCCC2(Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial) is also named as MCCB and belongs to the AccD/PCCB family. The putative 563-amino acid polypeptide has a calculated molecular mass of 61 kD and contains an N-terminal mitochondrial targeting sequence(PMID:11181649). It catalyzes carboxylation of 3 methylcrotonyl COA to form 3 methylglutaconyl-COA and is probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits. Defects in MCCC2 are the cause of methylcrotonoyl-CoA carboxylase 2 deficiency (MCC2D).

Publikationen

Species	Application	Title
human	WB,IHC	J Proteome Res; Profiling protein markers associated with lymph node metastasis in prostate cancer by DIGE-based proteomics analysis.; Authors - Pang Jun J
human	WB	Cancer Cell Int; MCCC2 promotes HCC development by supporting leucine oncogenic function.; Authors - Yu-Yan Chen ; KO Validated
	WB	Andrology; Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.; Authors - J Dai
human,mouse	WB,IF,CoIP	Mol Med; ECHDC2 inhibits the proliferation of gastric cancer cells by binding with NEDD4 to degrade MCCC2 and reduce aerobic glycolysis; Authors - Jiancheng He