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MCL1 Polyklonaler Antikörper

MCL1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(153)

Kat-Nr. : 16225-1-AP

Synonyme

Bcl2-L-3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, A431-Zellen, HEK-293T-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe, humanes Lebergewebe, K-562-Zellen, MCF-7-Zellen, Mausmilzgewebe, Raji-Zellen
Erfolgreiche IPRaji-Zellen
Erfolgreiche Detektion in IHChuman ovary cancer tissue
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen
Erfolgreiche Detektion in FC (Intra)Ramos-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16225-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA MCL1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MCL1 fusion protein Ag10397
Vollständiger Name myeloid cell leukemia sequence 1 (BCL2-related)
Berechnetes Molekulargewicht 350 aa, 37 kDa
Beobachtetes Molekulargewicht 35-40 kDa
GenBank-ZugangsnummerBC107735
Gene symbol MCL1
Gene ID (NCBI) 4170
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MCL1 antibody 16225-1-APProtokoll herunterladen
IHC protocol for MCL1 antibody 16225-1-APProtokoll herunterladenl
IF protocol for MCL1 antibody 16225-1-APProtokoll herunterladen
IP protocol for MCL1 antibody 16225-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism

Authors - Wang Jiahui
mouseIHC

Nat Commun

MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically.

Authors - Enkhtsetseg Munkhbaatar
humanWB

Nat Commun

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Authors - Di Hu
mouseWB

Nat Commun

IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation.

Authors - Senthil Kumaran Satyanarayanan
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Eya (Verified Customer) (07-06-2021)

the bands appear very clear

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: shTHP1