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MCL1 Polyklonaler Antikörper
MCL1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16225-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Jurkat-Zellen, A431-Zellen, HEK-293T-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe, humanes Lebergewebe, K-562-Zellen, MCF-7-Zellen, Mausmilzgewebe, Raji-Zellen | 
| Erfolgreiche IP | Raji-Zellen | 
| Erfolgreiche Detektion in IHC | human ovary cancer tissue Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | Ramos-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 11 publications below | 
| WB | See 135 publications below | 
| IHC | See 16 publications below | 
| IF | See 7 publications below | 
| IP | See 7 publications below | 
| CoIP | See 1 publications below | 
Produktinformation
16225-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA MCL1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | MCL1 fusion protein Ag10397 | 
| Vollständiger Name | myeloid cell leukemia sequence 1 (BCL2-related) | 
| Berechnetes Molekulargewicht | 350 aa, 37 kDa | 
| Beobachtetes Molekulargewicht | 35-40 kDa | 
| GenBank-Zugangsnummer | BC107735 | 
| Gene symbol | MCL1 | 
| Gene ID (NCBI) | 4170 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for MCL1 antibody 16225-1-AP | Protokoll herunterladen | 
| IHC protocol for MCL1 antibody 16225-1-AP | Protokoll herunterladenl | 
| IF protocol for MCL1 antibody 16225-1-AP | Protokoll herunterladen | 
| IP protocol for MCL1 antibody 16225-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Mol Cell The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism | ||
| Nat Commun MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically. | ||
| Nat Commun Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. | ||
| Nat Commun IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation. | ||
| EBioMedicine The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Eya (Verified Customer) (07-06-2021) | the bands appear very clear 
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