MCM4 Polyklonaler Antikörper

MCM4 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 13043-1-AP

Synonyme

CDC21, CDC54, EC:3.6.4.12, hCdc21, P1 CDC21

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  WB analysis of HeLa using 13043-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13043-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13043-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13043-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13043-1-AP

  IP result of anti-MCM4 (IP:13043-1-AP, 4ug; Detection:13043-1-AP 1:600) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human colon cancer using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 13043-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using MCM4 antibody (13043-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 13043-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using MCM4 antibody (13043-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HL-60-Zellen, humanes Lebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushodengewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below

Produktinformation

13043-1-AP bindet in WB, IHC, IF-P, IP, ELISA MCM4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCM4 fusion protein Ag3703
• Vollständiger Name: minichromosome maintenance complex component 4
• Berechnetes Molekulargewicht: 863 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC031061
• Gene symbol: MCM4
• Gene ID (NCBI): 4173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of MCM4 protein.

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly.; Authors - Nathalie Eisenhardt
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Phytother Res; Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma; Authors - Ronghui Fan
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali