MCM8 Polyklonaler Antikörper

MCM8 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16451-1-AP

Synonyme

C20orf154, MCM8, Minichromosome maintenance 8, REC

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Galerie der Validierungsdaten

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  WB analysis using 16451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16451-1-AP (MCM8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 16451-1-AP

  IP Result of anti-MCM8 (IP:16451-1-AP, 4ug; Detection:16451-1-AP 1:300) with NIH/3T3 cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16451-1-AP bindet in WB, IP, IF, CoIP, ELISA MCM8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCM8 fusion protein Ag8696
• Vollständiger Name: minichromosome maintenance complex component 8
• Berechnetes Molekulargewicht: 880 aa, 98 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC008830
• Gene symbol: MCM8
• Gene ID (NCBI): 84515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.

Publikationen

Species	Application	Title
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage.; Authors - Jen-Wei Huang
human	WB	Cell Death Dis; Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells.; Authors - Kang Liu
human	WB	Res Sq; Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB; Authors - Ananya Acharya
human	WB	Nat Commun; A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress; Authors - Wezley C. Griffin
human	WB,IF,CoIP	J Cell Biol; REC drives recombination to repair double-strand breaks in animal mtDNA; Authors - Anna Klucnika