MCM8 Polyklonaler Antikörper

MCM8 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 16451-1-AP

Synonyme

C20orf154, MCM8, Minichromosome maintenance 8, REC



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293T-Zellen, NIH/3T3-Zellen
Erfolgreiche IPNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16451-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MCM8 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MCM8 fusion protein Ag8696
Vollständiger Name minichromosome maintenance complex component 8
Berechnetes Molekulargewicht 880 aa, 98 kDa
Beobachtetes Molekulargewicht 90-95 kDa
GenBank-ZugangsnummerBC008830
Gene symbol MCM8
Gene ID (NCBI) 84515
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MCM8 antibody 16451-1-APProtokoll herunterladen
IP protocol for MCM8 antibody 16451-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human

Nat Commun

DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.

Authors - Jing Zhang
humanWB

Nat Commun

MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage.

Authors - Jen-Wei Huang
mouseIP

EMBO J

MCM8 interacts with DDX5 to promote R-loop resolution

Authors - Canxin Wen
humanWB

Res Sq

Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB

Authors - Ananya Acharya
humanWB

Cell Death Dis

Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells.

Authors - Kang Liu
humanWB

Nat Commun

A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress

Authors - Wezley C. Griffin