MCP-1/CCL2 Rekombinanter Antikörper

MCP-1/CCL2 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

230483A9

Kat-Nr. : CL594-98028

Synonyme

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

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  FC experiment of RAW 264.7 using CL594-98028

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL594-98028, Clone: 230483A9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using CL594-98028

  1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL594-98028, Clone: 230483A9) (red), or 0.25 ug Isotype Control (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL594-98028, Clone: 230483A9) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): mit LPS und Brefeldin A behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98028 bindet in FC (Intra) MCP-1/CCL2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: chemokine (C-C motif) ligand 2
• GenBank-Zugangsnummer: NM-011333
• Gene symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.