MCP1 Polyklonaler Antikörper

MCP1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF, ELISA, Blocking

Konjugation

Unkonjugiert

Publikationen(67)

Kat-Nr. : 25542-1-AP

Synonyme

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPMA, LPS and Brefeldin A treated THP-1 cells
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25542-1-AP bindet in IHC, IF, ELISA, Blocking MCP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Kaninchen
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MCP1 fusion protein Ag10601
Vollständiger Name chemokine (C-C motif) ligand 2
Berechnetes Molekulargewicht 99 aa, 11 kDa
Beobachtetes Molekulargewicht11 kDa
GenBank-ZugangsnummerBC009716
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 6347
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MCP1 antibody 25542-1-APProtokoll herunterladen
IHC protocol for MCP1 antibody 25542-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
humanIHC

Bone Res

Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome

Authors - Yanqiu Wang
human

J Neurol Neurosurg Psychiatry

Molecules of senescent glial cells differentiate Alzheimer's disease from ageing

Authors - Linbin Dai
humanIHC,IF

Cancer Lett

LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer

Authors - Hansen Lin
IF

J Hazard Mater

Polystyrene microplastics induced male reproductive toxicity in mice.

Authors - Haibo Jin
rabbitIHC

Theranostics

Macrophage targeted theranostic strategy for accurate detection and rapid stabilization of the inflamed high-risk plaque.

Authors - Joon Woo Song