MCP1 Polyklonaler Antikörper

MCP1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, Blocking, ELISA

Konjugation

Unkonjugiert

Publikationen

55

Kat-Nr. : 25542-1-AP

Synonyme

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

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Galerie der Validierungsdaten

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  WB analysis using 25542-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 25542-1-AP (MCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of THP-1 using 25542-1-AP

  1X10^6 HP-1 cells untreated (dashed lines) or treated with PMA (red) were intracellularly stained with 0.4 ug Anti-Human MCP1 (25542-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, LPS and Brefeldin A treated THP-1 cells
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: PMA treated THP-1 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 23 publications below
• IF: See 7 publications below

Produktinformation

25542-1-AP bindet in WB, IHC, IF, FC, Blocking, ELISA MCP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Kaninchen
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCP1 fusion protein Ag10601
• Vollständiger Name: chemokine (C-C motif) ligand 2
• Berechnetes Molekulargewicht: 99 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC009716
• Gene symbol: CCL2
• Gene ID (NCBI): 6347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Publikationen

Species	Application	Title
		ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
human		J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
	IF	J Hazard Mater; Polystyrene microplastics induced male reproductive toxicity in mice.; Authors - Haibo Jin
rabbit	IHC	Theranostics; Macrophage targeted theranostic strategy for accurate detection and rapid stabilization of the inflamed high-risk plaque.; Authors - Joon Woo Song
human	IHC	Theranostics; Reversing activity of cancer associated fibroblast for staged glycolipid micelles against internal breast tumor cells.; Authors - Yun Zhu
	IHC	Int J Cancer; Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma.; Authors - Qun Chen

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