ME1 Polyklonaler Antikörper

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• KD/KO Validated

ME1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (1)

Kat-Nr. : 16619-1-AP

Synonyme

EC:1.1.1.40, Malic enzyme 1, NADP-dependent malic enzyme, NADP-ME

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  WB analysis using 16619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 16619-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16619-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16619-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16619-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16619-1-AP

  IP result of anti-ME1 (IP:16619-1-AP, 4ug; Detection:16619-1-AP 1:2000) with mouse liver tissue lysate 2400 ug.

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  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16619-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ME1 antibody (16619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Mauslebergewebe, Maus-Plazenta-Gewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Produktinformation

16619-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ME1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ME1 fusion protein Ag9916
• Vollständiger Name: malic enzyme 1, NADP(+)-dependent, cytosolic
• Berechnetes Molekulargewicht: 572 aa, 64 kDa
• Beobachtetes Molekulargewicht: 55-64 kDa
• GenBank-Zugangsnummer: BC025246
• Gene symbol: ME1
• Gene ID (NCBI): 4199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
mouse	WB,IHC	Adv Sci (Weinh); Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury; Authors - Xuexian Fang
		Mol Cell; Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis.; Authors - Yahui Zhu
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chi (Verified Customer) (10-17-2024)

I have found the antibody in a publication (https://pubmed.ncbi.nlm.nih.gov/22789539/) and have tested the antibody myself. The antibody works great in WB with no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver cancer