MEF2C Polyklonaler Antikörper

MEF2C Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20326-1-AP

Synonyme

MEF2C, myocyte enhancer factor 2C

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20326-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20326-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20326-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 20326-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lymph using 20326-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 20326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50.

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  IHC staining of human heart using 20326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50.

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  IF Staining of mouse brain using 20326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maus-Dünndarmgewebe, Maushodengewebe, Rattenlymphgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20326-1-AP bindet in WB, IHC, IF, ELISA MEF2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEF2C fusion protein Ag13255
• Vollständiger Name: myocyte enhancer factor 2C
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• Beobachtetes Molekulargewicht: 45-70 kDa
• GenBank-Zugangsnummer: BC026341
• Gene symbol: MEF2C
• Gene ID (NCBI): 4208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.