MEFV Polyklonaler Antikörper

MEFV Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 24280-1-AP

Synonyme

Pyrin

---

• 

  WB analysis of human plasma using 24280-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24280-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 24280-1-AP

  IP result of anti-MEFV (IP:24280-1-AP, 4ug; Detection:24280-1-AP 1:5000) with THP-1 cells lysate 1820 ug.

• 

  IF Staining of A431 using 24280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MEFV antibody (24280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of A431 using 24280-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MEFV (24280-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, human plasma
• Erfolgreiche IP: THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

24280-1-AP bindet in WB, IF/ICC, FC (Intra), IP, ELISA MEFV und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEFV fusion protein Ag18000
• Vollständiger Name: Mediterranean fever
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC101511
• Gene symbol: MEFV
• Gene ID (NCBI): 4210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.

Publikationen

Species	Application	Title
mouse	WB,IP	Autophagy; Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes; Authors - Nivedita Hegdekar
human	WB	J Agric Food Chem; Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma; Authors - Yuxi Liu
rat	WB,IF	Neurochem Res; Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway; Authors - Hao Fang
rat	IF	Exp Ther Med; Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock.; Authors - Xin Jin
human	FC	PLoS Biol; Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever; Authors - Matthew S J Mangan
		Nature; FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation; Authors - Caroline Junqueira