MEKK3 Polyklonaler Antikörper

MEKK3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21072-1-AP

Synonyme

MAP3K3, MAPK/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK 3, MEKK3

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  WB analysis of mouse liver using 21072-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21072-1-AP (MEKK3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 21072-1-AP

  IP result of anti-MEKK3 (IP:21072-1-AP, 4ug; Detection:21072-1-AP 1:300) with mouse liver tissue lysate 7000ug.

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  IHC staining of human liver cancer using 21072-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21072-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

21072-1-AP bindet in WB, IHC, IF, IP, ELISA MEKK3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEKK3 fusion protein Ag15223
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 3
• Berechnetes Molekulargewicht: 657 aa, 74 kDa
• Beobachtetes Molekulargewicht: 71 kDa, 74 kDa
• GenBank-Zugangsnummer: BC090859
• Gene symbol: MEKK3
• Gene ID (NCBI): 4215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases. (PMID: 18206350).Cerebral cavernous malformations (CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.

Publikationen

Species	Application	Title
human,mouse	WB,IF,IP	Oncogene; MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function.; Authors - Jinqiu Lu
rat	WB	Sci Rep; Treadmill training improves lung function and inhibits alveolar cell apoptosis in spinal cord injured rats; Authors - Xianbin Wang
human	WB,IHC	Biomed Pharmacother; Buparlisib and ponatinib inhibit aggressiveness of cholangiocarcinoma cells via suppression of IRS1-related pathway by targeting oxidative stress resistance; Authors - Waleeporn Kaewlert