MELK Rekombinanter Antikörper

MELK Rekombinant Antikörper für FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230106D4

Kat-Nr. : 82893-1-RR

Synonyme

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

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  FC experiment of MCF-7 using 82893-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82893-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82893-1-RR bindet in FC (Intra), ELISA MELK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MELK fusion protein Ag1897
• Vollständiger Name: maternal embryonic leucine zipper kinase
• Berechnetes Molekulargewicht: 651 aa, 75 kDa
• GenBank-Zugangsnummer: BC014039
• Gene symbol: MELK
• Gene ID (NCBI): 9833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.