MEPCE Polyklonaler Antikörper

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MEPCE Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 11526-1-AP

Synonyme

Bin3 homolog, MEPCE, methylphosphate capping enzyme

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  WB analysis of MCF-7 using 11526-1-AP

  WB result of MEPCE antibody (11526-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPCE transfected MCF-7 cells.

• 

  WB analysis of HeLa using 11526-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11526-1-AP (MEPCE Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11526-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11526-1-AP (MEPCE Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11526-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11526-1-AP (MEPCE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11526-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11526-1-AP (MEPCE antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

11526-1-AP bindet in WB, IP, IHC, ELISA MEPCE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEPCE fusion protein Ag2092
• Vollständiger Name: methylphosphate capping enzyme
• Berechnetes Molekulargewicht: 689 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC018935
• Gene symbol: MEPCE
• Gene ID (NCBI): 56257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEPCE is a Component of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.

Publikationen

Species	Application	Title
human	WB	Dev Cell; Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation.; Authors - Kamaleldin E Elagib
zebrafish	WB,IP	Proc Natl Acad Sci U S A; 7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.; Authors - Barboric Matjaz M
human	WB	Nucleic Acids Res; 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.; Authors - Eilebrecht Sebastian S
human	WB	RNA Biol; 7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb.; Authors - Eilebrecht Sebastian S

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MIAO (Verified Customer) (09-17-2020)

good Ab with nice WB signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1K
• Cell Tissue Type: HCT cell