MEPE Polyklonaler Antikörper

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• KD/KO Validated

MEPE Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18804-1-AP

Synonyme

MEPE, OF45, Osteoblast/osteocyte factor 45

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 18804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18804-1-AP

  WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells.

• 

  WB analysis of A375 using 18804-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18804-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18804-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18804-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A2780-Zellen, A375-Zellen, HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18804-1-AP bindet in WB,ELISA MEPE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: matrix extracellular phosphoglycoprotein
• Berechnetes Molekulargewicht: 525 aa, 58 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 98 kDa
• GenBank-Zugangsnummer: BC128158
• Gene symbol: MEPE
• Gene ID (NCBI): 56955
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).