c-Met (Cytoplasmic) Rekombinanter Antikörper

c-Met (Cytoplasmic) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2G12

Kat-Nr. : 82904-1-RR

Synonyme

c-Met, MET, HGF receptor, EC:2.7.10.1, c Met

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  WB analysis of HepG2 using 82904-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82904-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 82904-1-RR

  WB result of c-Met (Cytoplasmic) antibody (82904-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met (Cytoplasmic) transfected HepG2 cells.

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  WB analysis of HEK-293 using 82904-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 82904-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-Met (Cytoplasmic) Recombinant antibody (82904-1-RR, Clone:2G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82904-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82904-1-RR against Human c-Met (Cytoplasmic) were performed. The affinity constant is 0.573 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82904-1-RR bindet in WB, FC (Intra), ELISA c-Met (Cytoplasmic) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: c-Met (Cytoplasmic) fusion protein Ag23140
• Vollständiger Name: met proto-oncogene (hepatocyte growth factor receptor)
• Berechnetes Molekulargewicht: 1390 aa, 155 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC130420
• Gene symbol: MET
• Gene ID (NCBI): 4233
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.