METAP2 Polyklonaler Antikörper

METAP2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17040-1-AP

Synonyme

MAP 2, MAP2, MetAP 2, METAP2, Methionine aminopeptidase 2, methionyl aminopeptidase 2, MNPEP, p67, p67eIF2, Peptidase M 2

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  WB analysis using 17040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17040-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17040-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17040-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 17040-1-AP

  IP result of anti-METAP2 (IP:17040-1-AP, 4ug; Detection:17040-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IF Staining of HepG2 using 17040-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17040-1-AP (METAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen, Maus-Pankreasgewebe, Maushodengewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17040-1-AP bindet in WB, IF/ICC, IP, ELISA METAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METAP2 fusion protein Ag10564
• Vollständiger Name: methionyl aminopeptidase 2
• Berechnetes Molekulargewicht: 478 aa, 53 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC013782
• Gene symbol: METAP2
• Gene ID (NCBI): 10988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MetAP2, also known as MAP2, P67EIF2 or p67, is one of the three known methionine aminopeptidases responsible for the removal of the N-terminal translation initiator methionine from newly synthesized proteins, which is a critical step in protein maturation (PMID: 27692546, 16568094). The enzyme binds two cobalt or manganese ions in its active site (PMID: 11524009, 12718546). As higher MetAP2 expression was observed in tumor (mesothelioma, neuroblastoma, and colorectal carcinoma) cells compared with normal cells, the enzyme serves as a target for anti-angiogenic compounds of natural origin, such as fumagillin and ovalicin (PMID: 11485930, 15927358, 15102683, 9224570). Selective inhibition of MetAP2 stops vascularization and tumor growth in animal models (PMID: 12118091). MetAP2 has 3 isoforms with molecular mass of 50-53 kDa and apparent molecular mass 67 kD.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Decoding the Function of Expansion Segments in Ribosomes.; Authors - Kotaro Fujii