METTL14 Polyklonaler Antikörper

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• KD/KO Validated

METTL14 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

88

Kat-Nr. : 26158-1-AP

Synonyme

KIAA1627, methyltransferase like 14, METTL14

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Galerie der Validierungsdaten

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  WB analysis using 26158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26158-1-AP (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 26158-1-AP

  WB result of METTL14 antibody (26158-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected Raji cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, NCCIT-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 23 publications below
• WB: See 73 publications below
• IHC: See 13 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• RIP: See 6 publications below

Produktinformation

26158-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA METTL14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL14 fusion protein Ag14325
• Vollständiger Name: methyltransferase like 14
• Berechnetes Molekulargewicht: 456 aa, 52 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC007449
• Gene symbol: METTL14
• Gene ID (NCBI): 57721
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.

Publikationen

Species	Application	Title
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	IF	Cell Res; The m6A methylome of SARS-CoV-2 in host cells.; Authors - Jun'e Liu ; KD Validated
	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
	WB	Neuron; Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System.; Authors - Yi-Lan Weng
human	RIP	Nat Commun; The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers.; Authors - Yaodong Zhang