METTL7A Monoklonaler Antikörper

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• KD/KO Validated

METTL7A Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A9B4

Kat-Nr. : 67905-1-Ig

Synonyme

AAM B, EC:2.1.1.348, EC:2.1.1.9, methyltransferase like 7A, Methyltransferase-like protein 7A

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  WB analysis using 67905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67905-1-Ig

  WB result of METTL7A antibody (67905-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

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  WB analysis of mouse lung using 67905-1-Ig

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, HepG2-Zellen, K-562-Zellen, LNCaP-Zellen, Mauslebergewebe, Mauslungengewebe, Kaninchenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

67905-1-Ig bindet in WB, IHC, ELISA METTL7A und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: METTL7A fusion protein Ag29986
• Vollständiger Name: methyltransferase like 7A
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC008180
• Gene symbol: METTL7A
• Gene ID (NCBI): 25840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.

Publikationen

Species	Application	Title
human	WB	Int J Oral Sci; METTL7A-mediated m6A modification of corin reverses bisphosphonates-impaired osteogenic differentiation of orofacial BMSCs; Authors - Yizhou Jin ; KD Validated