MFF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MFF Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

151

Rezensionen (2)

Kat-Nr. : 17090-1-AP

Synonyme

C2orf33, MFF, mitochondrial fission factor

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Galerie der Validierungsdaten

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  WB analysis using 17090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17090-1-AP (MFF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17090-1-AP

  IP result of anti-MFF(IP:17090-1-AP, 4ug; Detection:17090-1-AP 1:30000) with mouse brain tissue lysate 1680 ug.

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17090-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFF antibody (17090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 137 publications below
• IHC: See 4 publications below
• IF: See 23 publications below
• IP: See 4 publications below

Produktinformation

17090-1-AP bindet in WB, IP, IHC, IF, ELISA MFF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Eichh?rnchen, hamster, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MFF fusion protein Ag9990
• Vollständiger Name: mitochondrial fission factor
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC000797
• Gene symbol: MFF
• Gene ID (NCBI): 56947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Publikationen

Species	Application	Title
human	WB	Nature; Multiple dynamin family members collaborate to drive mitochondrial division.; Authors - Jason E Lee
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Nature; Correcting mitochondrial fusion by manipulating mitofusin conformations.; Authors - Antonietta Franco
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
monkey	WB	Science; ER tubules mark sites of mitochondrial division.; Authors - Jonathan R Friedman ; KD Validated
human	WB	Cell; CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity.; Authors - Philipp Hammerschmidt

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293 and HeLa

FH

JAE HO (Verified Customer) (04-22-2019)

I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: PC3/MDAMB231/LN229/MCF7/MCF10/DU145/LnCaP

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