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MGMT Monoklonaler Antikörper
MGMT Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
1H2C9
Kat-Nr. : 67476-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | U2OS-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, MOLT-4-Zellen, Raji-Zellen |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
| Erfolgreiche Detektion in FC (Intra) | Jurkat-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 2 publications below |
| IF | See 2 publications below |
Produktinformation
67476-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA MGMT und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | MGMT fusion protein Ag29936 |
| Vollständiger Name | O-6-methylguanine-DNA methyltransferase |
| Berechnetes Molekulargewicht | 22 kDa |
| Beobachtetes Molekulargewicht | 22 kDa |
| GenBank-Zugangsnummer | BC000824 |
| Gene symbol | MGMT |
| Gene ID (NCBI) | 4255 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for MGMT antibody 67476-1-Ig | Protokoll herunterladen |
| IF protocol for MGMT antibody 67476-1-Ig | Protokoll herunterladen |
| FC protocol for MGMT antibody 67476-1-Ig | Download protocol |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response. | ||
Cancer Discov Targeting Microglial Metabolic Rewiring Synergizes with Immune Checkpoint Blockade Therapy for Glioblastoma | ||
J Colloid Interface Sci Brain-targeted polymersomes mediating RNAi of STAT3 sensitize glioblastoma to temozolomide and immunotherapy |




