MGP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MGP Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (1)

Kat-Nr. : 10734-1-AP

Synonyme

GIG36, matrix Gla protein, MGLAP, MGP, NTI

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Galerie der Validierungsdaten

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse kidney using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (10734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (10734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Dünndarmgewebe, humanes Lymphomgewebe, humanes Mammahyperplasie-Gewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Produktinformation

10734-1-AP bindet in WB, IHC, IF, ELISA MGP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGP fusion protein Ag1091
• Vollständiger Name: matrix Gla protein
• Berechnetes Molekulargewicht: 103 aa, 13 kDa
• GenBank-Zugangsnummer: BC005272
• Gene symbol: MGP
• Gene ID (NCBI): 4256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix Gla protein (MGP), a vitamin K-dependent protein, is recognized as a calcification inhibitor in vascular tissue. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Publikationen

Species	Application	Title
mouse	IHC	Circulation; Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis.; Authors - Chang Kyunghwa K
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi
human	IHC	Arthritis Rheum; Juvenile dermatomyositis calcifications selectively displayed markers of bone formation.; Authors - Urganus Annette L AL
rat	WB	Aging (Albany NY); Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1.; Authors - Yao Chen
human	WB	FASEB J; 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes injury-induced vascular neointima formation in mice.; Authors - Shumin Guo
human	WB	Int J Obes (Lond); Using gene expression to predict the secretome of differentiating human preadipocytes.; Authors - Mutch D M DM

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (08-29-2023)

This antibody worked pretty well for IF on mouse liver frozen sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Liver

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