MICA/MICB Monoklonaler Antikörper

MICA/MICB Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

WB, FC

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

6D4

Kat-Nr. : 65161-1-Ig

Synonyme

MICA, MICB

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Galerie der Validierungsdaten

• 

  FC experiment of HeLa using 65161-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB (65161-1-Ig, Clone: 6D4) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

• 

  FC experiment of HeLa using 65161-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB (65161-1-Ig, Clone: 6D4) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

65161-1-Ig bindet in WB, FC MICA/MICB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Transfizierte MICA-exprimierende C1R-Zellen
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• GenBank-Zugangsnummer: BC016929
• Gene symbol: MICA
• Gene ID (NCBI): 100507436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Publikationen

Species	Application	Title
human	WB	J Immunother; BCMA-targeting Bispecific Antibody That Simultaneously Stimulates NKG2D-enhanced Efficacy Against Multiple Myeloma.; Authors - Yang Wang
human	WB	Int Immunopharmacol; Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway.; Authors - Hui-Yang Wu
human	WB	Cancers (Basel); circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis; Authors - Fei Liu