MIXL1 Polyklonaler Antikörper

MIXL1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 22772-1-AP

Synonyme

hMix, Homeobox protein MIXL1, Homeodomain protein MIX, MILD1, MIX, Mix.1 homeobox like protein, MIX1 homeobox like protein 1, MIXL, MIXL1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 22772-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 22772-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of H7ES using 22772-1-AP

  H7ES cells were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 22772-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 22772-1-AP (MIXL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 6 publications below

Produktinformation

22772-1-AP bindet in WB, IF, ELISA MIXL1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIXL1 fusion protein Ag18732
• Vollständiger Name: Mix1 homeobox-like 1 (Xenopus laevis)
• Berechnetes Molekulargewicht: 232 aa, 25 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC113441
• Gene symbol: MIXL1
• Gene ID (NCBI): 83881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mix1 homeobox-like 1 (MIXL1) Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. This antibody is specific to MIXL1.

Publikationen

Species	Application	Title
human	IF	Cell Stem Cell; Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.; Authors - Jian Hui Low
human	IF	Adv Healthc Mater; Spatial Stem Cell Fate Engineering via Facile Morphogen Localization; Authors - Gyuhyung Jin
		Cell Death Differ; Uric acid: a potent molecular contributor to pluripotent stem cell cardiac differentiation via mesoderm specification.; Authors - Bingbing Ke
human	IF	Int J Mol Sci; Porcine Kidney Organoids Derived from Naïve-like Embryonic Stem Cells; Authors - Meishuang Li
pig	IF	Front Cell Dev Biol; Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells; Authors - Meng Zhou
human	IF	STAR Protoc; Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.; Authors - Julie Bejoy