MKL1/MRTF-A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MKL1/MRTF-A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(39)

Rezensionen (1)

Kat-Nr. : 21166-1-AP

Synonyme

MKL1, KIAA1438, MAL, MKL/myocardin-like protein 1, MRTFA

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• 

  WB analysis using 21166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 21166-1-AP

  Immunofluorescent analysis of HeLa cells using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 18 publications below
• ChIP: See 5 publications below

Produktinformation

21166-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA MKL1/MRTF-A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MKL1/MRTF-A fusion protein Ag15240
• Vollständiger Name: megakaryoblastic leukemia (translocation) 1
• Berechnetes Molekulargewicht: 931 aa, 99 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC115039
• Gene symbol: MKL1
• Gene ID (NCBI): 57591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).

Publikationen

Species	Application	Title
mouse	IF	Cell Stem Cell; An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.; Authors - Allison P Kann
mouse	IF	Nat Cell Biol; Myc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation; Authors - Miguel Bernabé-Rubio
	IF	J Cell Biol; The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression.; Authors - Jaime Fernández-Barrera
mouse	IF	Elife; Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors.; Authors - Colin J Dinsmore
human	WB,IHC,IF,ChIP	Exp Mol Med; MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β; Authors - Fu Du ; KD Validated
human	WB	Oncotarget; The Wnt-β-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells.; Authors - Hongpeng He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Qingxue (Verified Customer) (06-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs