Melan-A Polyklonaler Antikörper

Melan-A Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 18472-1-AP

Synonyme

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, MLANA, Protein Melan A

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Galerie der Validierungsdaten

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA antibody) at dilution of 1:4000 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

18472-1-AP bindet in WB, IHC, ELISA Melan-A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Melan-A fusion protein Ag13346
• Vollständiger Name: melan-A
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13-20 kDa
• GenBank-Zugangsnummer: BC014423
• Gene symbol: MLANA
• Gene ID (NCBI): 2315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)

Publikationen

Species	Application	Title
mouse	IHC	Int J Mol Sci; The Role of Hedgehog Signaling in the Melanoma Tumor Bone Microenvironment; Authors - Karnoon Shamsoon
mouse	WB	Life Sci; Differentiation-inducing factor-1 reduces lipopolysaccharide-induced vascular cell adhesion molecule-1 by suppressing mTORC1-S6K signaling in vascular endothelial cells; Authors - Masaki Arioka

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chromogenic Kit from Leica Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Melanoma Tuor

Melan-A Antibody Immunohistochemistry validation (1:500 dilution) in Melanoma Tuor (Cat no:18472-1-AP)