MLH1 Monoklonaler Antikörper

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• KD/KO Validated

MLH1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F12C4

Kat-Nr. : 67350-1-Ig

Synonyme

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

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Galerie der Validierungsdaten

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  WB analysis using 67350-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67350-1-Ig (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67350-1-Ig

  WB result of MLH1 antibody (67350-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Caco-2-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67350-1-Ig bindet in WB, ELISA MLH1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MLH1 fusion protein Ag27723
• Vollständiger Name: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Berechnetes Molekulargewicht: 756 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa, 40-45 kDa
• GenBank-Zugangsnummer: BC006850
• Gene symbol: MLH1
• Gene ID (NCBI): 4292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.