MLPH Monoklonaler Antikörper

MLPH Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1A8D12

Kat-Nr. : 66092-1-Ig

Synonyme

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

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  WB analysis using 66092-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66092-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66092-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 66092-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

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  IHC staining of human skin cancer using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

• 

  IF Staining of HepG2 using 66092-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, Jurkat-Zellen, PC-3-Zellen, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

66092-1-Ig bindet in WB, IHC, IF/ICC, ELISA MLPH und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MLPH fusion protein Ag19286
• Vollständiger Name: melanophilin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC001653
• Gene symbol: MLPH
• Gene ID (NCBI): 79083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Publikationen

Species	Application	Title
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
human	WB	Mol Med Rep; Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes.; Authors - Shuhui Qi