Neprilysin/CD10 Polyklonaler Antikörper

Neprilysin/CD10 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 18008-1-AP

Synonyme

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe, humanes Nierengewebe, Rattennierengewebe
Erfolgreiche IPRaji-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Nierenzellkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Nierengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18008-1-AP bindet in WB, IHC, IF-P, IP, ELISA Neprilysin/CD10 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Neprilysin/CD10 fusion protein Ag12506
Vollständiger Name membrane metallo-endopeptidase
Berechnetes Molekulargewicht80aa,9 kDa; 750aa,85 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC106070
Gene symbol CD10
Gene ID (NCBI) 4311
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Neprilysin/CD10 antibody 18008-1-APProtokoll herunterladen
IHC protocol for Neprilysin/CD10 antibody 18008-1-APProtokoll herunterladenl
IF protocol for Neprilysin/CD10 antibody 18008-1-APProtokoll herunterladen
IP protocol for Neprilysin/CD10 antibody 18008-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Adv Mater

Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment

Authors - Yuewen Zhai
mouseWB

Adv Sci (Weinh)

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

Authors - Rui Zhou
humanWB

Brain

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu
human,mouseWB

Aging Cell

Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease

Authors - Guoxin Zhang
humanWB

Int J Biol Macromol

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Authors - Hui Zeng
mouseWB

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Authors - Yasuteru Inoue

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (10-04-2021)

calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.

  • Applications: Western Blot