Neprilysin/CD10 Polyklonaler Antikörper

Neprilysin/CD10 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 23898-1-AP

Synonyme

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

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  WB analysis of mouse kidney using 23898-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 23898-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 23898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Neprilysin/CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Ramos using 23898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using MME,CD10 antibody (23898-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, humanes Nierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

23898-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA Neprilysin/CD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neprilysin/CD10 fusion protein Ag20963
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC101658
• Gene symbol: CD10
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Publikationen

Species	Application	Title
human	IF	Cell Physiol Biochem; NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.; Authors - Xinliu Zeng
mouse	WB	Biochem Cell Biol; IL-6 induced enhanced clearance of proANP and ANP by insulin-degrading enzyme in T1DM mouse.; Authors - Caiyun Li
human	WB	J Biochem Mol Toxicol; miRNA-211-5p inhibition enhances the protective effect of hucMSC-derived exosome in Aβ1-40 -induced SH-SY5Y cells by increasing NEP expression; Authors - Huijing Chen
human	IF	J Transl Med; Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor; Authors - Yi Gong