MME,CD10 Monoklonaler Antikörper

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• KD/KO Validated

MME,CD10 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B9D9

Kat-Nr. : 60034-3-Ig

Synonyme

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 60034-3-Ig

  WB result of MME,CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MME,CD10 transfected HEK-293 cells.

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  WB analysis of pig kidney using 60034-3-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 60034-3-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (MME,CD10 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (MME,CD10 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of Ramos using 60034-3-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Raji using 60034-3-Ig

  1X10^6 Raji cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Nierengewebe, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Nierengewebe, humanes Tonsillengewebe, Mauslebergewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Ramos-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60034-3-Ig bindet in WB, IHC, FC, ELISA MME,CD10 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MME,CD10 fusion protein Ag0427
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC101658
• Gene symbol: MME
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C