Neprilysin/CD10 Monoklonaler Antikörper

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• KD/KO Validated

Neprilysin/CD10 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B9D9

Kat-Nr. : 60034-3-Ig

Synonyme

MME, Neprilysin, 4B9D9, Atriopeptidase, CALLA

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  WB analysis using 60034-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 60034-3-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60034-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60034-3-Ig

  WB result of Neprilysin/CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neprilysin/CD10 transfected HEK-293 cells.

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  WB analysis of Ramos using 60034-3-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 60034-3-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 60034-3-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Daudi-Zellen, HEK-293-Zellen, Mausnierengewebe, Hausschwein-Nierengewebe, Kaninchennierengewebe, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe, humanes Nierenzellkarzinomgewebe, humanes Magenkrebsgewebe, humanes Tonsillengewebe, Mausnierengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:25000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60034-3-Ig bindet in WB, IHC, ELISA Neprilysin/CD10 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Neprilysin/CD10 fusion protein Ag0427
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC101658
• Gene symbol: CD10
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).