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MMP13 Polyklonaler Antikörper

MMP13 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(603)

Kat-Nr. : 18165-1-AP

Synonyme

CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13, Matrix metalloproteinase-13



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen, Mauslebergewebe, Rattenlebergewebe
Erfolgreiche IPMCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Leberzirrhosegewebe, humanes Leberkarzinomgewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18165-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA MMP13 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Kaninchen, Maus, Ratte, Rind, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MMP13 fusion protein Ag12653
Vollständiger Name matrix metallopeptidase 13
Berechnetes Molekulargewicht 471 aa, 54 kDa
Beobachtetes Molekulargewicht 65-70 kDa, 50-55 kDa, 40 kDa
GenBank-ZugangsnummerBC074808
Gene symbol MMP13
Gene ID (NCBI) 4322
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MMP13 antibody 18165-1-APProtokoll herunterladen
IHC protocol for MMP13 antibody 18165-1-APProtokoll herunterladenl
IF protocol for MMP13 antibody 18165-1-APProtokoll herunterladen
IP protocol for MMP13 antibody 18165-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse,humanIF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanIF

Cell Stem Cell

A human organoid drug screen identifies α2-adrenergic receptor signaling as a therapeutic target for cartilage regeneration

Authors - Xiaocui Wei
ratIF

Bioact Mater

Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repair

Authors - Changjian Lin
mouse,humanWB,IF

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
mouseWB

Nat Commun

Osteocyte-derived extracellular vesicles mediate the bone-to-cartilage crosstalk and promote osteoarthritis progression

Authors - Na Liu
mouse,humanWB,IF

Adv Sci (Weinh)

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

Authors - Bin Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xie (Verified Customer) (05-13-2021)

Good IHC staining for Rat MMP13.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: rat knee joints
MMP13 Antibody Immunohistochemistry validation (1:100 dilution) in rat knee joints (Cat no:18165-1-AP)
FH

Hongxue (Verified Customer) (01-12-2021)

clear wb band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
MMP13 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:18165-1-AP)