MMP26 Polyklonaler Antikörper

MMP26 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 18087-1-AP

Synonyme

Matrix metalloproteinase 26, matrix metallopeptidase 26, Matrilysin-2, Matrilysin 2, Endometase

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  WB analysis of SKOV-3 using 18087-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 18087-1-AP (MMP26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18087-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18087-1-AP (MMP26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18087-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18087-1-AP (MMP26 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 18087-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 18087-1-AP (MMP26 Antibody) at dilution of 1:50.

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  IHC staining of human prostate cancer using 18087-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 18087-1-AP (MMP26 Antibody) at dilution of 1:50.

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  IF Staining of SKOV-3 using 18087-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 18087-1-AP (MMP26 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, HEK-293-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

18087-1-AP bindet in WB, IHC, IF/ICC, ELISA MMP26 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP26 fusion protein Ag12806
• Vollständiger Name: matrix metallopeptidase 26
• Berechnetes Molekulargewicht: 262 aa, 30 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC101541
• Gene symbol: MMP26
• Gene ID (NCBI): 56547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP26(matrix metallopeptidase 26) may hydrolyze collagen type IV, fibronectin, fibrinogen, beta-casein, type I gelatin and alpha-1 proteinase inhibitor.MMP26 is expressed in placenta and uterus and is widely expressed in malignant tumors from different sources as well as in diverse tumor cell lines(PMID: 10987280).The proenzyme without the signal peptide has a calculated molecular mass of 29 kDa and the processed enzyme has a calculated molecular mass of 19 kDa(PMID:15574774).This antibody is specific to MMP26.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage; Authors - Sarah Abdullah
human	IHC	J Huazhong Univ Sci Technolog Med Sci; MMP26: A potential biomarker for prostate cancer.; Authors - Teng Cheng