MMP28 Polyklonaler Antikörper

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• KD/KO Validated

MMP28 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 18237-1-AP

Synonyme

MM28, Matrix metalloproteinase-28, Matrix metalloproteinase 28, matrix metallopeptidase 28, Epilysin

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  WB analysis of A549 using 18237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18237-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 18237-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 18237-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP28 antibody (18237-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Kolongewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hautkrebsgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

18237-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MMP28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP28 fusion protein Ag12980
• Vollständiger Name: matrix metallopeptidase 28
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC002631
• Gene symbol: MMP28
• Gene ID (NCBI): 79148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

Publikationen

Species	Application	Title
mouse	WB,IHC	Phytomedicine; Targeting the NF-κB p65-MMP28 axis: Wogonoside as a novel therapeutic agent for attenuating podocyte injury in diabetic nephropathy; Authors - Xiandeng Li
mouse	IHC,IF	Cell Mol Life Sci; Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.; Authors - Kyoung-In Cho
human	WB	FASEB J; Drastic induction of MMP-7 by cortisol in the human amnion: implications for membrane rupture at parturition.; Authors - Lu-Yao Wang
human	WB	Am J Pathol; CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.; Authors - Zhang Min M
mouse	WB,IF	Dis Model Mech; Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.; Authors - Kyoung-In Cho
human	IHC	Am J Pathol; Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis.; Authors - Anne M Manicone