MMP3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MMP3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

34

CloneNo.

1F5A9

Kat-Nr. : 66338-1-Ig

Synonyme

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 66338-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66338-1-Ig

  WB result of MMP3 antibody (66338-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP3 transfected HeLa cells.

• 

  WB analysis of human heart using 66338-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66338-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66338-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66338-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66338-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66338-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66338-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Caco-2-Zellen, COLO 320-Zellen, fetales humanes Hirngewebe, Hausschwein-Herzgewebe, Hausschwein-Hirngewebe, HeLa-Zellen, humanes Herzgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Produktinformation

66338-1-Ig bindet in WB, IHC, IF, ELISA MMP3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MMP3 fusion protein Ag12359
• Vollständiger Name: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• Berechnetes Molekulargewicht: 477 aa, 54 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BC074869
• Gene symbol: MMP3
• Gene ID (NCBI): 4314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Publikationen

Species	Application	Title
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger
mouse	IHC	J Nanobiotechnology; Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression; Authors - Yunhao Wang
human	WB,IHC	Acta Pharmacol Sin; Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.; Authors - Jia-Wei Li
human	WB	Aging (Albany NY); ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.; Authors - Jingyu Ding
mouse	WB	EBioMedicine; Allergy-induced systemic inflammation impairs tendon quality.; Authors - Christine Lehner
human	WB	Front Cell Dev Biol; Reducing Inflammation and Vascular Invasion in Intervertebral Disc Degeneration via Cystathionine-γ-Lyase Inhibitory Effect on E-Selectin.; Authors - Haoran Xu

Verwandte Produkte

RAC1 Monoclonal antibody

66122-1-Ig

JNK Monoclonal antibody

66210-1-Ig

TIMP1 Polyclonal antibody

16644-1-AP