MMS19 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MMS19 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2H6D8

Kat-Nr. : 66049-1-Ig

Synonyme

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 66049-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66049-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66049-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66049-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66049-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66049-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 66049-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66049-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66049-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 66049-1-Ig

  IP Result of anti-MMS19 (IP:66049-1-Ig, 4ug; Detection:66049-1-Ig 1:2000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 66049-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66049-1-Ig(MMS19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 66049-1-Ig

  Immunofluorescent analysis of fixed Hela cells using 66049-1-Ig (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Nierengewebe, NIH/3T3-Zellen, Rattenhirngewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66049-1-Ig bindet in WB, IP, IHC, IF, ELISA MMS19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MMS19 fusion protein Ag8960
• Vollständiger Name: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1030 aa, 113 kDa
• Beobachtetes Molekulargewicht: 103 kDa, 38 kDa
• GenBank-Zugangsnummer: BC006575
• Gene symbol: MMS19
• Gene ID (NCBI): 64210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

Publikationen

Species	Application	Title
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling ; KD Validated
human	WB	Mol Cell; An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis.; Authors - Adarsh K Mayank
human	WB	Cancer Biomark; MMS19 as a potential predictive marker of adjuvant chemotherapy benefit in resected non-small cell lung cancer.; Authors - Julien Adam ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang