MND1 Polyklonaler Antikörper

MND1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11636-1-AP

Synonyme

GAJ, MND1

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Galerie der Validierungsdaten

• 

  WB analysis of human liver using 11636-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11636-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11636-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11636-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11636-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11636-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11636-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11636-1-AP

  IP Result of anti-MND1 (IP:11636-1-AP, 4ug; Detection:11636-1-AP 1:600) with mouse liver tissue lysate 6000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, humanes Lungengewebe, humanes Nierengewebe, Mauslebergewebe, MCF-7-Zellen
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11636-1-AP bindet in WB, IP, IF, ELISA MND1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MND1 fusion protein Ag2210
• Vollständiger Name: meiotic nuclear divisions 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 205 aa, 24 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC032142
• Gene symbol: MND1
• Gene ID (NCBI): 84057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Oncotarget; A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).; Authors - Matthew Tsang
human	WB	Med Oncol; Meiotic nuclear divisions 1 promotes proliferation and metastasis in hepatocellular carcinoma and is a potential diagnostic and therapeutic target gene; Authors - Kai Tan ; KD Validated