MON1A Polyklonaler Antikörper

MON1A Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 23772-1-AP

Synonyme

MON1 homolog A (yeast), MON1A, SAND1

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Galerie der Validierungsdaten

• 

  WB analysis using 23772-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23772-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23772-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23772-1-AP bindet in WB,ELISA MON1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MON1A fusion protein Ag6900
• Vollständiger Name: MON1 homolog A (yeast)
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa and 45 kDa
• GenBank-Zugangsnummer: BC047022
• Gene symbol: MON1A
• Gene ID (NCBI): 84315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MON1A is a cytosolic protein that peripherally associates with the ER. MON1A plays an important role in membrane trafficking through the secretory apparatus. MON1A affects different steps in the secretory pathway including endoplasmic reticulum-to-Golgi traffic. MON1A encodes several isoforms with MW aboout 73, 62, 55, 45 and 34 kDa.

Publikationen

Species	Application	Title
	WB	Cell Death Dis; TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.; Authors - Jia Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Q (Verified Customer) (06-01-2021)

It can produce a stronger band a little higher than 55. But its identity is not quite sure as in human cells Mon1A is supposed to be around 73 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 cell lysate

MON1A Antibody Western Blot validation (1:1000 dilution) in HEK293 cell lysate (Cat no:23772-1-AP)