MORF4L2 Polyklonaler Antikörper

MORF4L2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14710-1-AP

Synonyme

Mortality factor 4-like protein 2, mortality factor 4 like 2, MORF-related gene X protein, MORFL2, MORF related gene X protein

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  WB analysis of HeLa using 14710-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 14710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14710-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14710-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 14710-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MORF4L2 antibody (14710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14710-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14710-1-AP (MORF4L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, humanes Lebergewebe, MCF-7-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

14710-1-AP bindet in WB, IHC, IF/ICC, ELISA MORF4L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MORF4L2 fusion protein Ag6404
• Vollständiger Name: mortality factor 4 like 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC056899
• Gene symbol: MORF4L2
• Gene ID (NCBI): 9643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Risk stratification based on DNA damage-repair-related signature reflects the microenvironmental feature, metabolic status and therapeutic response of breast cancer; Authors - Chunzhen Li