MPHOSPH6 Polyklonaler Antikörper

MPHOSPH6 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (2)

Kat-Nr. : 10695-1-AP

Synonyme

M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6

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  WB analysis of MCF-7 using 10695-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10695-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 10695-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10695-1-AP

  IP result of anti-MPHOSPH6 (IP:10695-1-AP, 4ug; Detection:10695-1-AP 1:500) with HeLa cells lysate 3000 ug.

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  IHC staining of human breast cancer using 10695-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10695-1-AP bindet in WB, IP, IHC, ELISA MPHOSPH6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPHOSPH6 fusion protein Ag1080
• Vollständiger Name: M-phase phosphoprotein 6
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC005242
• Gene symbol: MPHOSPH6
• Gene ID (NCBI): 10200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Cell Prolif; The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis.; Authors - Rui-Rui Peng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody that specifically recognise Mphosph6

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:10695-1-AP)

FH

Ralf (Verified Customer) (08-04-2022)

10µg of the total mouse protein were loaded on the gel. Antibodies also stain the ladder a bit.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in (Cat no:10695-1-AP)