MPO Monoklonaler Antikörper

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• KD/KO Validated

MPO Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

4C11F6

Kat-Nr. : CL594-66177

Synonyme

myeloperoxidase, EC:1.11.2.2, 4C11F6

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  IF Staining of human liver using CL594-66177

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 MPO antibody (CL594-66177, Clone: 4C11F6 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL594-66177

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®594 MPO antibody (CL594-66177, Clone: 4C11F6 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human appendicitis using CL594-66177

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®594 MPO antibody (CL594-66177, Clone: 4C11F6 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HL-60 using CL594-66177

  1X10^6 HL-60 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human MPO (CL594-66177, Clone:4C11F6) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Appendizitis-Gewebe, humanes Lebergewebe
• Erfolgreiche Detektion in FC (Intra): HL-60-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-66177 bindet in IF-P, FC (Intra) MPO und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MPO fusion protein Ag17564
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	IF	Thromb Haemost; Neutrophil Extracellular Traps: Potential Prothrombotic State Markers and Therapeutic Targets for Atrial Fibrillation; Authors - Xing Liu