MR1 Polyklonaler Antikörper

MR1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 13260-1-AP

Synonyme

HLALS, MR1

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Galerie der Validierungsdaten

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  WB analysis of THP-1 cells using 13260-1-AP

  WB result of MR1 antibody (13260-1-AP, 1:250) with IP samples of THP.1-MR1 cells, by Claudia Gonzalez-Lopez, MRC Human Immunology Unit, University of Oxford. (Green MR1 protein).

• 

  IF Staining of THP-1 cells using 13260-1-AP

  IF result of MR1 antibody (13260-1-AP, 1:50) with THP.1-MR1 cells, by Claudia Gonzalez-Lopez, MRC Human Immunology Unit, University of Oxford.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IF: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:100-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

13260-1-AP bindet in WB, IF, FC, ELISA MR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MR1 fusion protein Ag3849
• Vollständiger Name: major histocompatibility complex, class I-related
• Berechnetes Molekulargewicht: 341 aa, 39 kDa
• GenBank-Zugangsnummer: BC012485
• Gene symbol: MR1
• Gene ID (NCBI): 3140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Ligand-dependent downregulation of MR1 cell surface expression.; Authors - Mariolina Salio
mouse	IF	Carbohydr Polym; Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy.; Authors - Tingting Guo
human	WB	J Biol Chem; The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.; Authors - Corinna A Kulicke
human	FC	Am J Pathol; Bile from Patients with Primary Sclerosing Cholangitis Contains Mucosal-Associated Invariant T-Cell Antigens.; Authors - Laura Valestrand

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tina (Verified Customer) (08-31-2020)

- PAGE was loaded with 80ug total protein - PVDF membrane blocked with milk - first AB dilution 1:250 (stored at -20°C, I could reuse the solution multiple times) - secondary AB I used HRP goat anti-rabbit and a high sensitive ECl to develop the Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: cell lines K562, primary liver cells