MRPL11 Rekombinanter Antikörper

MRPL11 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242768G1

Kat-Nr. : 85109-4-RR

Synonyme

242768G1, CGI 113, CGI-113, L11mt, Large ribosomal subunit protein uL11m

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  IF Staining of HepG2 using 85109-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 85109-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 85109-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MRPL11 Recombinant antibody (85109-4-RR, Clone:242768G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85109-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85109-4-RR against Human MRPL11 were performed. The affinity constant is 14.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85109-4-RR bindet in IF/ICC, FC (Intra), ELISA MRPL11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MRPL11 fusion protein Ag7899
• Vollständiger Name: mitochondrial ribosomal protein L11
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005002
• Gene symbol: MRPL11
• Gene ID (NCBI): 65003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.