MRPL24 Polyklonaler Antikörper

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• KD/KO Validated

MRPL24 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16224-1-AP

Synonyme

MRP-L24, MRP L24, MRP L18, Large ribosomal subunit protein uL24m, L24mt

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  WB analysis of HEK-293 using 16224-1-AP

  WB result of MRPL24 antibody (16224-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL24 transfected HEK-293 cells.

• 

  WB analysis using 16224-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16224-1-AP (MRPL24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16224-1-AP (MRPL24 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16224-1-AP

  IP result of anti-MRPL24 (IP:16224-1-AP, 4ug; Detection:16224-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

16224-1-AP bindet in WB, IP, ELISA MRPL24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL24 fusion protein Ag9277
• Vollständiger Name: mitochondrial ribosomal protein L24
• Berechnetes Molekulargewicht: 216 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC016700
• Gene symbol: MRPL24
• Gene ID (NCBI): 79590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
	WB	J Biol Chem; The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.; Authors - Sofia Zaganelli
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita
human	WB	3 Biotech; MRPL24 drives breast cancer metastasis and stemness by targeting c-MYC, BRD4, and STAT3; Authors - Abdul Jamil Khan
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia