MRPL48 Polyklonaler Antikörper

MRPL48 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 14677-1-AP

Synonyme

CGI 118, CGI118, CGI-118, HSPC290, L48mt

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  WB analysis of Jurkat using 14677-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14677-1-AP (MRPL48 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14677-1-AP (MRPL48 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14677-1-AP (MRPL48 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 14677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14677-1-AP (MRPL48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14677-1-AP (MRPL48 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 14677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MRPL48 antibody (14677-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

14677-1-AP bindet in WB, IHC, IF/ICC, ELISA MRPL48 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL48 fusion protein Ag6387
• Vollständiger Name: mitochondrial ribosomal protein L48
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 20-21 kDa
• GenBank-Zugangsnummer: BC009764
• Gene symbol: MRPL48
• Gene ID (NCBI): 51642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL48, also named as CGI-118, or HSPC290 is a 212 amino acid protein, which localizes in the Mitochondrion. MRPL48 is a component of the large subunit of the mitochondrial ribosome, including two RNA molecules and, at least, 70 proteins among which 47 proteins for the large subunit. mitochondrial ribosomal protein helps in protein synthesis within the mitochondrion.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.; Authors - Brooke C Wilson
human	WB	Nucleic Acids Res; Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.; Authors - Surovtseva Yulia V YV
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
bovine	IF	Int J Mol Sci; Low Expression of Mitofusin 1 Gene Leads to Mitochondrial Dysfunction and Embryonic Genome Activation Failure in Ovine-Bovine Inter-Species Cloned Embryos; Authors - Shanshan Wu
mouse	WB	Cell Rep; MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.; Authors - Jakob D Busch
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim