MRPS18B Polyklonaler Antikörper

MRPS18B Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(59)

Kat-Nr. : 16139-1-AP

Synonyme

28S ribosomal protein S18-2, mitochondrial, C6orf14, HSPC183, MRP S18 2, MRP S18 b

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  WB analysis using 16139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16139-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 16139-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16139-1-AP

  IP result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of A549 using 16139-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug MRPS18B Polyclonal antibody (16139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, MCF-7-Zellen, Mauslebergewebe, PC-3-Zellen, Rattenherzgewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 53 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

16139-1-AP bindet in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA MRPS18B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS18B fusion protein Ag9198
• Vollständiger Name: mitochondrial ribosomal protein S18B
• Berechnetes Molekulargewicht: 258 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC005373
• Gene symbol: MRPS18B
• Gene ID (NCBI): 28973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.

Publikationen

Species	Application	Title
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
	WB	Mol Cell; A kinetic dichotomy between mitochondrial and nuclear gene expression processes; Authors - Erik McShane
	WB,CoIP	Cell Host Microbe; Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition.; Authors - Joseph Verdi
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nat Struct Mol Biol; Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.; Authors - Xingqian Zhang
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia