MRPS25 Polyklonaler Antikörper

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• KD/KO Validated

MRPS25 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 15277-1-AP

Synonyme

Small ribosomal subunit protein mS25, S25mt, RPMS25, MRP-S25, MRP S25

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  WB analysis using 15277-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15277-1-AP (MRPS25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15277-1-AP

  WB result of MRPS25 antibody (15277-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS25 transfected HepG2 cells.

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  IP experiment of HEK-293 using 15277-1-AP

  IP result of anti-MRPS25 (IP:15277-1-AP, 4ug; Detection:15277-1-AP 1:500) with HEK-293 cells lysate 1600ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15277-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15277-1-AP (MRPS25 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15277-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15277-1-AP (MRPS25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IP: See 1 publications below

Produktinformation

15277-1-AP bindet in WB, IHC, IP, ELISA MRPS25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS25 fusion protein Ag7395
• Vollständiger Name: mitochondrial ribosomal protein S25
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC003590
• Gene symbol: MRPS25
• Gene ID (NCBI): 64432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.

Publikationen

Species	Application	Title
mouse	WB	PLoS Genet; Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction.; Authors - Tara R Richman
human	WB	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
human	WB	Biochem J; Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.; Authors - Dennerlein Sven S
human	WB	Biochim Biophys Acta; Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.; Authors - Serre Valérie V
mouse	WB	Methods Mol Biol; Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.; Authors - Benedetta Ruzzenente
human	WB,IP	Nucleic Acids Res; BOLA3 and NFU1 link mitoribosome iron-sulfur cluster assembly to multiple mitochondrial dysfunctions syndrome; Authors - Hui Zhong