MRPS25 Monoklonaler Antikörper

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• KD/KO Validated

MRPS25 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3B10B6

Kat-Nr. : 67903-1-Ig

Synonyme

DKFZp313H0817, FLJ00023, MRP S25, MRPS25, RPMS25, S25mt

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  WB analysis using 67903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67903-1-Ig

  WB result of MRPS25 antibody (67903-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS25 transfected HepG2 cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67903-1-Ig bindet in WB, ELISA MRPS25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MRPS25 fusion protein Ag7846
• Vollständiger Name: mitochondrial ribosomal protein S25
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC003590
• Gene symbol: MRPS25
• Gene ID (NCBI): 64432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.

Publikationen

Species	Application	Title
human	WB	Cell Rep; A microscopy-based screen identifies cellular kinases modulating mitochondrial translation; Authors - Roya Yousefi