MRPS26 Polyklonaler Antikörper

MRPS26 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 15989-1-AP

Synonyme

C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt

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  WB analysis of HepG2 using 15989-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15989-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15989-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15989-1-AP

  IP result of anti-MRPS26 (IP:15989-1-AP, 4ug; Detection:15989-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

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  IHC staining of human skin cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15989-1-AP bindet in WB, IHC, IP, ELISA MRPS26 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS26 fusion protein Ag8797
• Vollständiger Name: mitochondrial ribosomal protein S26
• Berechnetes Molekulargewicht: 205 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC013018
• Gene symbol: MRPS26
• Gene ID (NCBI): 64949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
mouse	WB	Life Sci Alliance; Mitochondrial stress response triggered by defects in protein synthesis quality control.; Authors - Uwe Richter
human	WB	Clin Exp Immunol; Cultured lymphocytes' mitochondrial genome integrity is not altered by cladribine; Authors - Elina Järvinen