MSH2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MSH2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Kat-Nr. : 15520-1-AP

Synonyme

MSH 2

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  WB analysis of HeLa using 15520-1-AP

  WB results of MSH2 (15520-1-AP, 1:5000) antibody with si-Control and si-MSH2 transfected HeLa cells..

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  WB analysis using 15520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15520-1-AP

  IP result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human stomach cancer using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (15520-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 15520-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 (15520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen, SW480-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Tonsillengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 15 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

15520-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA MSH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSH2 fusion protein Ag7835
• Vollständiger Name: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100 kDa-105 kDa
• GenBank-Zugangsnummer: BC021566
• Gene symbol: MSH2
• Gene ID (NCBI): 4436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Publikationen

Species	Application	Title
mouse	IHC	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
human	WB	Leukemia; Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia; Authors - Ji-Yuan Teng ; KO Validated
human	WB	J Immunother Cancer; KALRN mutations promote antitumor immunity and immunotherapy response in cancer.; Authors - Mengyuan Li
human	IHC	Cancer Lett; Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination; Authors - Shujia Chen
human	WB, IHC	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai ; KD Validated
human	WB	Cancer Prev Res (Phila); Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.; Authors - Jin Peng P