MSH6 Monoklonaler Antikörper

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• KD/KO Validated

MSH6 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

2E10B2

Kat-Nr. : 66172-1-Ig

Synonyme

2E10B2, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP

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  WB analysis of HEK-293 using 66172-1-Ig

  WB result of MSH6 antibody (66172-1-Ig, 1:1000) with si-Control and si-MSH6 transfected HEK293 cells.

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  WB analysis of HEK-293 using 66172-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66172-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66172-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66172-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66172-1-Ig

  IP result of anti-MSH6 (IP:66172-1-Ig, 4ug; Detection:66172-1-Ig 1:600) with HEK-293 cells lysate 1800ug.

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  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Ramos using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 66172-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH6 antibody (66172-1-Ig, Clone: 2E10B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  FC experiment of HEK-293 using 66172-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug MSH6 Monoclonal antibody (66172-1-Ig, Clone:2E10B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Ramos-Zellen; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 8 publications below

Produktinformation

66172-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA MSH6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MSH6 fusion protein Ag12645
• Vollständiger Name: mutS homolog 6 (E. coli)
• Berechnetes Molekulargewicht: 153 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC004246
• Gene symbol: MSH6
• Gene ID (NCBI): 2956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Publikationen

Species	Application	Title
mouse	IHC	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
human	IHC	Cancer Lett; Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination; Authors - Shujia Chen
	IHC	Cancer Immunol Res; Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.; Authors - Qijing Wu
mouse	WB	J Med Chem; CJ2: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane; Authors - Renming Fan
human	WB	Respir Res; TMPRSS2 is a tumor suppressor and its downregulation promotes antitumor immunity and immunotherapy response in lung adenocarcinoma; Authors - Zhixian Liu
human	WB,IHC	Mol Carcinog; The mechanism of RGS5 regulating gastric cancer mismatch repair protein; Authors - Zhenwei Yang